Numbering Code	U-PHA00 3C906 PJ86	Year/Term	2022 ・ Second semester
Number of Credits	4	Course Type	Practical training
Target Year	3rd year students or above	Target Student
Language	Japanese	Day/Period
Instructor name	KAKIZAWA SHO (Graduate School of Pharmaceutical Sciences Associate Professor) SHIN HYE-WON (Graduate School of Pharmaceutical Sciences Associate Professor) HIRASAWA AKIRA (Graduate School of Pharmaceutical Sciences Associate Professor) KATOH YOHEI (Graduate School of Pharmaceutical Sciences Senior Lecturer) AKIBA HIROKI (Graduate School of Pharmaceutical Sciences Assistant Professor) ICHIMURA ATSUHIKO (Graduate School of Pharmaceutical Sciences Assistant Professor) ITO TAKAHIRO (Institute for Life and Medical Sciences Professor) KATOH HIRONORI (Graduate School of Biostudies Associate Professor) KANDA HIROSHI (Graduate School of Biostudies Associate Professor) Ayuna Hattori (Institute for Frontier Life and Medical Sciences Associate Professor) OHUE RYUJI (Graduate School of Biostudies Assistant Professor) OKIGAWA SAYUMI (Institute for Life and Medical Sciences Assistant Professor) KENKYO MATSUURA (Institute for Life and Medical Sciences Assistant Professor)
Outline and Purpose of the Course	The purpose of this training is to acquire the knowledge and skills necessary to carry out basic experiments in biochemistry and genomic drug-discovery science, and to understand the basic concepts of pharmaceutical science in the life sciences.
Course Goals	Learn biochemical experimental methods of working with enzymes Learn experimental methods of basic molecular genetics and biochemistry and understand recombinant DNA technologies Learn applied experimental methods of biochemistry involving cell-cell competition and communication Learn applied experimental methods of biochemistry using animal tissues and cells Learn applied experimental methods of biochemistry using cultured cells Learn experimental methods of genome analysis
Schedule and Contents	Training sessions (Department in charge) ○ Biochemistry training I (Biological Chemistry) Biochemical experiments on proteins (enzymes) (1) Substrate specificity of enzymatic reactions (2) pH dependence of enzymatic reactions (3) Kinetics and inhibition mechanisms of enzymatic reactions ○ Biochemistry training II (Cell Fate Dynamics and Therapeutics/Institute for Frontier Life and Medical Sciences) Performing experiments to understand molecular genetics and biochemistry using genes for fluorescent proteins (1) Transforming Escherichia coli with a foreign DNA (2) Analyzing recombinant DNAs and proteins ○ Biochemistry training III (Genetics/Graduate School of Biostudies) Genetic experiments with Drosophila (1) Analysis of signal-transduction pathways using Drosophila (2) Analysis of gene-expression control using Drosophila ○ Biochemistry training IV (Physiological Chemistry) Applied biochemistry experiments using animal tissues and cells (1) Removal of animal tissues and preparation of homogenates (2) Fractionation of intracellular organelles and marker tests (3) Observation and identification of intracellular organelles using a fluorescence microscope ○ Biochemistry training V (Molecular Neurobiology/Graduate School of Biostudies) Applied biochemistry experiments using cultured cells (1) Gene transfer into cultured cells (2) Observation of cytoskeletons with a fluorescence microscope ○ Genomic drug-discovery science training (Pharmacogenomics and Biopharmaceutical Chemistry) Genome-analysis experiments (1) Genomic DNA polymorphism analysis (2) Introduction to bioinformatics
Evaluation Methods and Policy	Attitude score and report score are evaluated using the following ratios; Biochemistry training I, Genomic drug-discovery science training: attitude score 40%, report score 60% Biochemistry training II, III, IV, V: attitude score 50%, report score 50% Details will be announced at the beginning of each training session by instructors.
Course Requirements	None
Study outside of Class (preparation and review)	Specified by the instructor in charge of each training session.
Textbooks	Textbooks/References	“Practice book”
References, etc.	『新生化学実験講座』 (Tokyo Kagaku Dojin), 『生物薬科学実験講座』 (Hirokawa Shoten), 『微生物学実習提要』 (Maruzen)