Numbering Code	U-PHA00 3C901 PJ86	Year/Term	2022 ・ First semester
Number of Credits	4	Course Type	Practical training
Target Year	3rd year students or above	Target Student
Language	Japanese	Day/Period
Instructor name	HOSHINO MASARU (Graduate School of Pharmaceutical Sciences Associate Professor) SUGIYAMA NAOYUKI (Graduate School of Pharmaceutical Sciences Associate Professor) OGAWA HARUO (Graduate School of Pharmaceutical Sciences Associate Professor) PAN DONGAING (Graduate School of Pharmaceutical Sciences Assistant Professor)
Outline and Purpose of the Course	After learning basic experimental data handling and statistical processing through all the different kinds of training, students will learn about basic measurement and analytical methods related to analysis and physical chemistry, namely spectroscopy, neutralization titration, separation analysis, mass spectrometry, electrochemical measurement (membrane surface potential/conductivity/electromotive force), X-ray crystal structure analysis, and protein structure visualization.
Course Goals	1. Learn experimental methods related to analytical chemistry, physical chemistry, and structural biology. 2. Correctly interpret the experimental data obtained.
Schedule and Contents	(Common) 1. Introductory lecture (basic statistical processing) (Molecular & Cellular Bioanalysis) 2. Absorption analysis: Basic absorption analysis and drug quantification (1) 3. Absorption analysis: Basic absorption analysis and drug quantification (2) 4. Neutralization titration: Calculation of factors and true reagent concentration 5. Back titration: Quantification of aspirin using titration method 6. HPLC: Basics and applications of HPLC, derivation of the decomposition-rate constant of aspirin (1) 7. HPLC: Basics and applications of HPLC, derivation of the decomposition-rate constant of aspirin (2) 8. Mass Spectrometer: Basics and applications of mass spectrometry, proteome-analysis method (1) 9. Mass Spectrometer: Basics and applications of mass spectrometry, proteome-analysis method (2) (Biophysical Chemistry) 10. NMR: 1H-NMR spectrum measurement, light-water elimination method 11. NMR: 1H-NMR spectrum measurement, two-dimensional spectrum analysis 12. Membrane binding and the surface potential of drugs: preparation of liposomes, measurement of the membrane partition coefficient of drugs, Gouy-Chapman theory (1) 13. Membrane binding and the surface potential of drugs: preparation of liposomes, measurement of the membrane partition coefficient of drugs, Gouy-Chapman theory (2) 14. Conductivity: Measurement of the ion hydration number, acetic-acid dissociation constant, critical micelle concentration (1) 15. Conductivity: Measurement of ion hydration number, acetic-acid dissociation constant, critical micelle concentration (2) 16. Concentration cell: Measurement of the electromotive force of a silver ion concentration cell and the average activity coefficient of silver nitrate (Structural Biology) 17. Protein crystallization (1) 18. Protein crystallization (2) 19. X-ray diffraction experiment, protein conformation determination (1) 20. X-ray diffraction experiment, protein conformation determination (2) 21. X-ray diffraction experiment, protein conformation determination (3) 22. Visualizing and drawing the three-dimensional structure of proteins (1) 23. Visualizing and drawing the three-dimensional structure of proteins (2) 24. Visualizing and drawing the three-dimensional structure of proteins (3)
Evaluation Methods and Policy	Evaluation is based on the following: normal score (training attitude) 50%; and reports 50%. In the reports, students will have to show that they have mastered the theory and experimental methods needed to conduct experiments in analytical chemistry, physical chemistry, and structural biology. They must also correctly interpret and discuss the experimental data obtained. In relation to training attitude, the evaluation will cover attendance status, prior preparation for training, and experimental attitude, which may influence the final grade.
Course Requirements	None
Study outside of Class (preparation and review)	Students are expected to read the textbook in advance to check and understand the procedures used in actual experiments.
Textbooks	Textbooks/References	Textbook for the course
References, etc.	Introduced during the course