Environmental Organic Micropollutants Analysis Lab.

Numbering Code G-ENG03 6F468 SJ16 Year/Term 2022 ・ Intensive, year-round
Number of Credits 2 Course Type Seminar
Target Year Target Student
Language Japanese Day/Period Intensive
Instructor name SHIMIZU YOSHIHISA (Graduate School of Engineering Professor)
MATSUDA TOMONARI (Graduate School of Engineering Associate Professor)
Outline and Purpose of the Course There is increasing concern about proper risk evaluation and management of hazardous chemicals such as dioxins and endocrine disruptors. To manage this problem, it is necessary to understand analytical methods and toxicity of those hazardous chemicals. In this class, lectures and experiments will be carried out about chromatography, bioassays and mass spectrometry.
Course Goals Understand about principle and practical techniques of chromatography. Understand about principle of several bioassays.
Schedule and Contents HPLC -How to separate it-,3times,Learn about principle and practice of HPLC separation. How do you choose columns, solvents and detectors? How to improve peak separation?
Fractionation and Purification by using HPLC,3times,Learn about practical techniques of fractionation and purification using HPLC.
LC/MS/MS,5times,Learn about principle and practice of LC/MS/MS analysis. Understand about 3 different scan modes, full scan, daughter scan and MRM. How to make an analytical method in a refined way for substances of your interest.
Bioassays,4times,Lecture about several bioassays which are used for evaluation of environmental toxicity, and discuss about how to identify toxic compounds in environment by using HPLC in combination with bioassays.
Evaluation Methods and Policy It is required to attend all 3 days for lectures and experiments. Attendance and reports are considered for grading.
Course Requirements None
Study outside of Class (preparation and review) We hope active participation of students. It is welcome that patticipants additionally try to analyze the sample their own interest.
Textbooks Textbooks/References Handouts are distributed.
References, etc. Daniel C. Harris: Quantitative Chemical Analysis ISBN-13: 978-1-4292-3989-9
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