Laboratory Practice in Basic Biofunctional Chemistry

Numbering Code G-PHA01 74210 PJ86 Year/Term 2022 ・ Irregular, year-round
Number of Credits 1 Course Type Practical training
Target Year 1st and 2nd year master's students Target Student
Language Japanese Day/Period
Instructor name FUTAKI SHIROH (Institute for Chemical Research Professor)
IMANISHI MIKI (Institute for Chemical Research Associate Professor)
Outline and Purpose of the Course This course aims to teach students the basic experiment methods and teaching methods they need for research in molecular biology, cell biology, and the chemistry of biological functions and physiological functions. During the course, students will design practical training and plan experiments relating to biofunctional chemistry, prepare and conduct preliminary experiments prior to practical training, and provide guidance during practical training. In addition to the basic experiment methods and approaches for research on biofunctional chemistry, the course aims to teach students experiment planning skills and teaching methods.
Course Goals To learn the basic experiment methods and approaches to conduct research on biofunctional chemistry, and also learn experiment planning skills and teaching methods.
Schedule and Contents It is intended that 3-21 weeks of classes will be spent on each of the following subjects:
1. Design and planning of practical training (Shiroh Futaki)
Design practical training and plan experiments on biofunctional chemistry
2. Preparation and preliminary experiments (Shiroh Futaki)
Prepare practical training content on biofunctional chemistry to explain to undergraduate students and carry out preliminary experiments to assess the validity of experiment plans.
3. Instruction in practical training (Miki Imanishi)
(1) Preparations using the protein genetic engineering method
(2) DNA recognition of transcription factor proteins and measurement of transcriptional activity
(3) Basic handling and microscopic observation of cells
(4) Chemical synthesis of peptides and chemical modification of proteins
(5) Interaction analysis of biomembranes, and peptides and proteins
4. Presentations and discussion of practical training results (Shiroh Futaki)
Presentation and discussion of practical training results in biofunctional chemistry
Evaluation Methods and Policy Students will be comprehensively evaluated based on practical training attendance, development and planning of practical training, preparation and preliminary experiments, and the quality and appropriateness of their instruction in practical training.
Course Requirements None
Study outside of Class (preparation and review) Students should attend the course with a thorough understanding of their master's program research topic and the state and issues of related fields.
Textbooks Textbooks/References Not used
References, etc. Others; printouts will be distributed where appropriate.
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