Laboratory Practice in Basic Physiological Chemistry

Numbering Code G-PHA01 74206 PJ86 Year/Term 2022 ・ Irregular, year-round
Number of Credits 1 Course Type Practical training
Target Year 1st and 2nd year master's students Target Student
Language Japanese Day/Period
Instructor name NAKAYAMA KAZUHISA (Graduate School of Pharmaceutical Sciences Professor)
KIMURA IKUO (Graduate School of Biostudies Professor)
SHIN HYE-WON (Graduate School of Pharmaceutical Sciences Associate Professor)
KATOH HIRONORI (Graduate School of Biostudies Associate Professor)
KATOH YOHEI (Graduate School of Pharmaceutical Sciences Senior Lecturer)
Outline and Purpose of the Course (Aim) This course aims to teach students basic experiment methods and approaches in hygiene chemistry, physiological chemistry, and cell biology, and equip them with experiment planning skills and teaching methods.
(Summary) Students will design practical training and plan experiments relating to hygiene chemistry, physiological chemistry, and cell biology in Specialized Training for Pharmacy 3 for Faculty of Pharmaceutical Sciences undergraduates. They will prepare and conduct preliminary training prior to practical training and guide undergraduate students during practical training.
Course Goals To learn teaching methods by designing and leading in experiments.
Schedule and Contents 1-15: The following practical training will be held:
Design and planning of practical training
・Design practical training relating to hygiene chemistry, physiological chemistry, and cell biology
・ Plan experiments relating to hygiene chemistry, physiological chemistry, and cell biology
Preparation and preliminary experiments
・ Prepare practical training relating to hygiene chemistry, physiological chemistry, and cell biology for the purposes of explanation
・ Carry out preliminary experiments to assess the validity of experiment planning relating to hygiene chemistry, physiological chemistry, and cell biology
Instruction in practical training
・ Experiment on isozyme-subunit association in lactate dehydrogenase
・ Subcellular organelle fractionation, marker molecule-based evaluation, and microscopic observation using GFP fusion proteins
・ Subcellular localization determination of proteins using GFP fusion proteins
・ Primary cultures of rat cerebral cortex neurons
・ Differentiation experiments on nerve cells using nerve growth factors
Presentations and discussion of practical training results
・Presentations and discussion of practical training results relating to hygiene chemistry, physiological chemistry, and cell biology
Evaluation Methods and Policy Students will be comprehensively evaluated based on practical training attendance, development and planning of practical training, preparation and preliminary experiments, and the quality and appropriateness of their instruction in practical training.
Course Requirements None
Study outside of Class (preparation and review) For students to lead practical training, they will need to prepare in advance, understand the background and wider picture of the practical training content, and go over it again following the practical training.
Textbooks Textbooks/References Practical training in pharmacy
References, etc. Others; printouts will be distributed where appropriate.
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