Laboratory Practice in Basic Molecular Biology
| Numbering Code | G-PHA01 74204 PJ86 | Year/Term | 2022 ・ Irregular, year-round | |
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| Number of Credits | 1 | Course Type | Practical training | |
| Target Year | 1st and 2nd year master's students | Target Student | ||
| Language | Japanese | Day/Period | ||
| Instructor name |
TAKESHIMA HIROSHI (Graduate School of Pharmaceutical Sciences Professor) IKUTA KOUICHI (Institute for Life and Medical Sciences Professor) ITO TAKAHIRO (Institute for Life and Medical Sciences Professor) KAKIZAWA SHO (Graduate School of Pharmaceutical Sciences Associate Professor) |
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| Outline and Purpose of the Course | In this course, students will design lab-course experiments related to metabolic biochemistry, cellular physiology, microbiology and immunology In practical training for undergraduates, students will have roles as instructors to contribute to the preparation of experimental materials, demonstration of experimental manipulation and support for summarization of experimental data. | |||
| Course Goals | To learn basic experiments in molecular biology and to develop leadership skills by supporting the lab-course practice of undergraduates. | |||
| Schedule and Contents |
(1) (2) Planning of experimental training To plan experiments related to metabolic biochemistry, cellular physiology, microbiology and immunology for lab-course training. (3) (4) Pilot experiments To prepare required materials for the experimental training planned for undergraduates, and to conduct the pilot experiments. (5)~(14) Instruction of lab-course training Bacterial cDNA expression experiment Enzyme reaction for kinetics analysis Electrophoresis of proteins and nucleic acids Gram staining for bacterial classification Generation of drug-resistant bacteria Analysis of bacterial UV sensitivity Virus infection in cultured cells |
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| Evaluation Methods and Policy | Students will be comprehensively evaluated based on practical training attendance, practical training design and planning, preparation and preliminary experiments, and the quality and appropriateness of their instruction in practical training. | |||
| Course Requirements | None | |||
| Study outside of Class (preparation and review) | There are other experiment methods that can be used for protein quantification and electrophoresis besides those used in this course. Using databases to learn about the features of the different experiment methods will help students further develop their experiment implementation skills. | |||
| Textbooks | Textbooks/References | Practical training in pharmacy | ||
| References, etc. | Others; printouts will be distributed where appropriate. | |||
