Laboratory Practice in Basic Analytical Chemistry and Structural Biology
| Numbering Code | G-PHA01 74202 PJ86 | Year/Term | 2022 ・ Irregular, year-round | |
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| Number of Credits | 1 | Course Type | Practical training | |
| Target Year | 1st and 2nd year master's students | Target Student | ||
| Language | Japanese | Day/Period | ||
| Instructor name |
MATSUZAKI KATSUMI (Graduate School of Pharmaceutical Sciences Professor) KATOU HIROAKI (Graduate School of Pharmaceutical Sciences Professor) HOSHINO MASARU (Graduate School of Pharmaceutical Sciences Associate Professor) PAN DONGAING (Graduate School of Pharmaceutical Sciences Assistant Professor) OGAWA HARUO (Graduate School of Pharmaceutical Sciences Associate Professor) KAWANO KENICHI (Graduate School of Pharmaceutical Sciences Assistant Professor) |
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| Outline and Purpose of the Course |
(Aim) This course aims to teach students approaches to basic experiment methods in physical chemistry and structural biology, as well as experiment planning skills and teaching methods. (Summary) In this course, students will plan practical training and experiments relating to physical chemistry and structural biology for Faculty of Pharmaceutical Sciences undergraduates in Specialized Training for Pharmacy 1, make preparations for and conduct preliminary experiments prior to beginning practical training, and guide students during practical training. |
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| Course Goals | To develop the ability to plan and conduct suitable basic experiments relating to physical chemistry and structural biology. To develop the ability to guide undergraduate students in preparation and preliminary experiments prior to beginning practical training as well as during practical training. | |||
| Schedule and Contents |
1. NMR:1H-NMR spectra measurement and the light water elimination method 2. Membrane binding of drugs and surface potential: Preparation of liposome, membrane partition coefficient measurement of drugs, and Gouy-Chapman theory (1) 3. Membrane binding of drugs and surface potential : Preparation of liposome, membrane partition coefficient measurement of drugs, and Gouy-Chapman theory (2) 4. Conductivity: Measurement of ion hydration number, acetic acid disassociation constant, and critical micelle concentration (1) 5. Conductivity: Measurement of ion hydration number, acetic acid disassociation constant, and critical micelle concentration (2) 6. Concentration cell: Measurement of electromotive force in silver ion concentration cells and average activity coefficient of silver nitrate (1) 7. Concentration cell: Measurement of electromotive force in silver ion concentration cells and average activity coefficient of silver nitrate (2) 8. Crystallization of proteins (1) 9. Crystallization of proteins (2) 10. Crystallization of proteins (3) 11. Crystallization of proteins (4) 12. Protein conformation determination, and visualization and depiction of conformation (1) 13. Protein conformation determination, and visualization and depiction of conformation (2) 14. Protein conformation determination, and visualization and depiction of conformation (3) 15. Protein conformation determination, and visualization and depiction of conformation (4) |
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| Evaluation Methods and Policy | Students will be comprehensively evaluated based on practical training attendance, development and planning of practical training, preparation and preliminary experiments, and the quality and appropriateness of their instruction in practical training. | |||
| Course Requirements | None | |||
| Study outside of Class (preparation and review) | Students should make preparations and conduct preliminary experiments to enable specialized training in pharmaceutical sciences to be carried out appropriately. Once the practical training is complete, students should arrange the training room appropriately for the next practical training session. | |||
| Textbooks | Textbooks/References | Others; practical training in pharmacy | ||
| References, etc. | Others; printouts will be distributed where appropriate. | |||
