Research in Molecular and Cellular Bioanalysis
| Numbering Code | G-PHA01 73203 EJ86 | Year/Term | 2022 ・ Irregular, year-round | |
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| Number of Credits | 4 | Course Type | Experiment | |
| Target Year | 1st and 2nd year master's students | Target Student | ||
| Language | Japanese | Day/Period | ||
| Instructor name |
ISHIHAMA YASUSHI (Graduate School of Pharmaceutical Sciences Professor) SUGIYAMA NAOYUKI (Graduate School of Pharmaceutical Sciences Associate Professor) |
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| Outline and Purpose of the Course |
(Aim) Through laboratory experiments, this course aims to teach students how to approach research and draft research plans; students will also learn experimental techniques, data analysis methods, and problem-solving skills. (Summary) In this course, we develop new technologies for proteomic analysis using mass spectrometry and microscale separation science and informatics, and conduct applied research on the proteomics-based drug discovery and cellular function analysis. |
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| Course Goals | Through the development of new technologies in proteomic analysis, proteomic drug discovery, and cellular functional analysis, this course teaches students how to approach and tackle research, and how to draft research plans; the course also teaches students about experimental techniques and data analysis methods, and develops their problem-solving skills. | |||
| Schedule and Contents |
Each of the following subjects will be given 2-3 weeks: ・Research on the development of new separation in LC-MS proteomics ・Research on the reannotation of genomic structures through shotgun proteomics ・Research on the development of new phosphopeptide enrichment in phosphoproteomics ・Research on intracellular signal transduction network analysis using phosphoproteomics ・Research on functional analysis and the development of new analysis of lysine acetylation modified proteomics ・Research on the application of simultaneous analysis of proteomes in drug discovery ・Research on the application of phosphoproteome analysis in drug discovery ・Research on the application of proteome analysis in cell biology ・Research on LC proteomics ・Research on the development of new quantitative systems using proteomic mass spectrometry |
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| Evaluation Methods and Policy | Students will be evaluated according to their attitude and approach to research (80%) and their research findings (20%). | |||
| Course Requirements | None | |||
| Study outside of Class (preparation and review) | Students should double check their work after the day's experiment is done to ensure there is nothing missing in their experiment notes. They should also ensure that their control samples are suitable and that the number of repetitions is sufficient before beginning their experiments. | |||
| Textbooks | Textbooks/References | Instructed during class | ||
| References, etc. | Discussed in class | |||
