Laboratory Course in Forest Biochemistry 1?
| Numbering Code | G-AGR01 5BC68 EB80 | Year/Term | 2022 ・ Year-round |
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| Number of Credits | 5 | Course Type | Experiment |
| Target Year | Target Student | ||
| Language | Japanese and English | Day/Period | |
| Instructor name |
HONDA YOICHI (Graduate School of Agriculture Professor) SAKAMOTO MASAHIRO (Graduate School of Agriculture Associate Professor) NAKAZAWA TAKEHITO (Graduate School of Agriculture Assistant Professor) KAWAUCHI MORIYUKI (Graduate School of Agriculture Program-Specific Associate Professor) |
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| Outline and Purpose of the Course | Experimental analysis of forest biomass and its utilization will be directed using biochemical and molecular biological strategy. Fundamental and application aspects of current studies in the related field will be given. | ||
| Course Goals |
It is required to be able to do the following during the year-round participation: 1. understand the principles for the experiments in the related field 2. discuss the experimental results in a correct and objective manner 3. write his/her own opinions in scientific sentences. |
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| Schedule and Contents |
Research theme will be directed for each student by the instructor. Examples are: 1. gene expression control in lignin-degrading fungi 2. protein secretion pathway in mushroom and carbohydrate modification of secreted proteins 3. degradation mechanism of woody biomass by basidiomycetes 4. molecular biology of flowering control in higher plants such as bamboo 5. physiological function and synthesis of the flavonoids in trees 6. mechanism for synthesis of cell wall components in plant. During the course, students will learn various experimental procedures including 1. Genetic analysis Forward and reverse genetics, gene cloning, gene expression, hybridization, recombinant DNA, genetic transformation, gene knock out, RNAi, CRISPR/Cas9 etc. 2. Protein analysis Purification, enzymatic kinetics, electrophoresis, chromatography, carbohydrate analysis of glycoproteins, over-expression etc. 3. Chemical analysis NMR, mass spectrometry, component analysis of decayed wood etc. 4. Data analysis statistical analysis, and scientific and logical consideration. |
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| Evaluation Methods and Policy |
Refer to current year's 'Guide to Degree Programs' for attainment levels of evaluation. Attendance(50%), idea(10%), originality(10%), forwardness(10%), comprehension(10%) and results(10%) will be considered for the evaluation of each student. |
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| Course Requirements | General knowleges in biochemistry, molecular biology, botany and mushroom science are required. | ||
| Study outside of Class (preparation and review) | Preparation for knowledge of the general and up-to-date experimental techniques are required. | ||
