Experimental Course of Applied Structural Biology 2
| Numbering Code | G-AGR03 6CC38 EB79 | Year/Term | 2022 ・ Year-round |
|---|---|---|---|
| Number of Credits | 6 | Course Type | Experiment |
| Target Year | Target Student | ||
| Language | Japanese | Day/Period | |
| Instructor name | MIZUTANI KIMIHIKO (Graduate School of Agriculture Assistant Professor) | ||
| Outline and Purpose of the Course | We are studying about structure-function relationship of proteins and enzymes based on their structures determined by X-ray crystallography. During the course of experiments, the students will learn the fundamental knowledge and technique about proteomics and structural biology. | ||
| Schedule and Contents |
Our special concern is structure/function relationship of food related enzymes and proteins such as amylase, protease, transglutaminase, glutaminase, ovalbumin, soybean protein, and ovotransferrin. The basic method of this experimental course is as follows. 1. Protein expression, purification and crystallization. Purification and crystallization are performed after target proteins and their mutant are expressed abundantly in expression system of E. coli and yeast. 2. Structure determination and refinement by X-ray crystallography. Students learn the method of X-ray crystallography by using in-house detector and synchrotron facilities. 3. Functional analysis of the target proteins based on the structures determined by X-ray crystallography. This is usually performed by the analysis of the complex structures with protein ligand such as substrate. Mutants of protein/ligand complex are also analyzed. We can recognize protein conformational change important for their functions. We also estimate the protein/ligand complex structures by simulation techniques. 4. Design of new functional proteins based on their structures. Based on the protein structure and conformational change, we can design new functional proteins with increased biological activities and stabilities. 5. Preparation of large single crystals for the purpose of neutron diffraction experiment. In order to determine the position of hydrogen in protein molecule, we are trying neutron diffraction experiments. |
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| Evaluation Methods and Policy | days attended and research achievements | ||
| Course Requirements | Students should have the basic knowledge about biochemistry and protein chemistry. | ||
