Special Laboratory Work in Pomology2
| Numbering Code | G-AGR01 6AC35 EB78 | Year/Term | 2022 ・ Year-round |
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| Number of Credits | 5 | Course Type | Experiment |
| Target Year | Target Student | ||
| Language | Japanese | Day/Period | |
| Instructor name |
TAO RIYUUTAROU (Graduate School of Agriculture Professor) YAMANE HISAYO (Graduate School of Agriculture Associate Professor) NISHIYAMA SOICHIRO (Graduate School of Agriculture Assistant Professor) |
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| Outline and Purpose of the Course | This course is developed to acquire several experimental techniques needed to fruit tree research. Morphological studies, tissue culture, instrumental analysis using GC, HPLC, electrophoresis, and DNA sequencer will be performed in this course. | ||
| Course Goals | Master a variety of experimental techniques related to pomology. | ||
| Schedule and Contents |
Tissue and cell culture techniques of fruit trees and development of transgenic strains (5 sessions) Students will examine efficient propagation methods using fruit tree culture techniques, and consider the possibility of developing fruit tree breeding methods using transgenic strains. Analyses of factors that regulate fruit fleshiness on fruit trees (5 sessions) Students will observe tissues and cells and conduct gene analysis to identify factors that regulate fruit fleshiness on fruit trees. Analyses of incompatibility in fruit trees (5 sessions) Using stone fruits, students will analyze proteins associated with incompatibility, and determine the mechanisms of gene expression regulation in the proteins. Determination of fruit tree dormancy (5 sessions) In order to determine the molecular mechanism of the dormancy of persimmons and stone fruits, students will analyze the dynamics of proteins and mRNA in winter buds. Investigation of molecular markers of fruit traits (5 sessions) Students will investigate the molecular markers linked to factors that determine whether a persimmon fruit is sweet or astringent, examine their effectiveness and analyze the markers’ properties. Gender analysis of fruit trees (5 sessions) Students will analyze the gender control of Diospyros plants at the gene level and examine how gender is controlled genetically. |
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| Evaluation Methods and Policy | Evaluation will be based on reports and attendance. | ||
| Course Requirements | Basic knowledge in pomology is required. Familiarity with basic experiment techniques is also desirable. | ||
| Study outside of Class (preparation and review) | Students are required to read relevant research papers and experiment reference books to gain an in-depth understanding of the latest experimental techniques. | ||
