Laboratory Course in Marine Molecular Microbiology2
| Numbering Code | G-AGR04 6DC43 EJ81 | Year/Term | 2022 ・ Year-round |
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| Number of Credits | 6 | Course Type | Experiment |
| Target Year | Target Student | ||
| Language | Japanese | Day/Period | |
| Instructor name |
YOSHIDA TAKASHI (Graduate School of Agriculture Professor) KAMIKAWA RYOUMA (Graduate School of Agriculture Associate Professor) |
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| Outline and Purpose of the Course |
Microorganisms are ubiquitously and abundantly present in all aquatic ecosystems represented by fresh waters and marine waters from coastal to oceanic waters as well as surface to deep sea waters. Some of them favors even extreme aquatic habitats. Further, viruses coexisting with them exceed in number. Interaction between microorganisms and viruses has considerable impact on the aquatic biogeochemical cycles. This course emphasizes research on ecology and physiology, biochemistry, omics and application of aquatic microorganisms and viruses to the study of their roles in global nutrient cycling and their evolution and construction of a platform towards production of oil independent energy and new materials. |
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| Course Goals |
1. Design a research plan, develop an experimental framework, and analyze the results by bringing together the techniques mastered in Laboratory Course in Marine Molecular Microbiology 1; 2. Master the ability to discover new information from experiment results and develop new scientific perspectives on life and experimental techniques. |
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| Schedule and Contents |
1) Ecological research on aquatic microbes (five weeks) Students will elucidate the community composition and metabolic genes of microbes in an environment by conducting metagenomic analysis, understand their relationships with chemical and physical factors, and assess the impact of microbes on the environment. 2) Isolation and cultivation of aquatic microbes and physiological research (five weeks) Students will isolate and cultivate micro algae and environmental microbes that are difficult to culture, characterize the optimal conditions for growing these organisms from physiological and ecological perspectives, and search for valuable genes in genetic isolates by conducting a genome sequencing analysis. 3) Molecular biochemical research on aquatic microbes (five weeks) Students will learn how to analyze the structures and functions of target genes and how to conduct a biochemical analysis on the manifested genetically modified proteins, and conduct a basic study for their applications. |
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| Evaluation Methods and Policy | Evaluation will be based on interim report sessions held 4 times a year, research papers and their presentations. Refer to '2019 Guide to Degree Programs' for attainment levels of evaluation. | ||
| Course Requirements | Knowledge of microbiology, biochemistry, molecular biology, and microbial ecology is required. | ||
| Study outside of Class (preparation and review) | Experiment reference books and original research papers will be assigned for reading depending on the students’ research progress. | ||
