Special Laboratory Work in Plant Breeding1
| Numbering Code | G-AGR01 5AC30 EB78 | Year/Term | 2022 ・ Year-round |
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| Number of Credits | 5 | Course Type | Experiment |
| Target Year | Target Student | ||
| Language | Japanese | Day/Period | |
| Instructor name |
NASUDA SHUUHEI (Graduate School of Agriculture Professor) TERAISHI MASAYOSHI (Institute for Liberal Arts and Sciences Associate Professor) YOSHIKAWA TAKANORI (Graduate School of Agriculture Assistant Professor) |
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| Outline and Purpose of the Course | Lectures and training for the phenotyping agronomic trais, genotyping using molecular markers, and the basic techniques of tissues culture and gene expression analysis. | ||
| Course Goals | Students will develop the techniques needed to breed, observe and analyze research materials, so that they can engage in their research projects independently. | ||
| Schedule and Contents |
10 weeks of classes and seminars are planned for each topic. 1) Gene expression analysis: lectures and experiments related to gene expression analyses using quantitative PCR methods, the Northern blotting technique, and microarray methods. 10 weeks 2) Tissue culture: lectures and experiments related to transient gene expression analysis through embryonic gene modification and protoplast culture. 10 weeks 3) DNA/proteins: lectures and experiments related to the molecular biological techniques necessary for the analysis of gene functions, such as gene cloning, gene structural analysis, and protein analysis. 10 weeks |
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| Evaluation Methods and Policy | Grading will be based on a comprehensive assessment of attendance and commitment to projects. | ||
| Course Requirements |
It is desirable that students have completed Plant Breeding. Basic knowledge of molecular biology and genetics is required. |
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| Study outside of Class (preparation and review) | Basic knowledge of plant nutrition, plant physiology, and soil science is needed for breeding stress-resistant plants, and basic knowledge of plant pathology and applied entomology are required for breeding pest-resistant plants. Students must keep abreast of the latest information in the fields related to their research themes. | ||
