Special Laboratory Work in Vegetable and
| Numbering Code | G-AGR01 6AC33 EJ78 | Year/Term | 2022 ・ Year-round |
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| Number of Credits | 5 | Course Type | Experiment |
| Target Year | Target Student | ||
| Language | Japanese | Day/Period | |
| Instructor name |
DOI MOTOAKI (Graduate School of Agriculture Professor) TANAKA YOSHIYUKI (Graduate School of Agriculture Associate Professor) OHNO SHO (Graduate School of Agriculture Assistant Professor) |
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| Outline and Purpose of the Course | The course aims to learn advanced laboratory skills in vegetable and ornamental horticultural science; experimental design and statistical analysis, genetic analysis, quality analysis, environmental control and measurements, morphological observation, microscopy, and etc. | ||
| Course Goals | Master the laboratory skills needed to conduct research in vegetable and ornamental horticulture and other related fields, and apply them in an innovative way. | ||
| Schedule and Contents |
1. Theory and methods of growth analysis (2 sessions) Students will learn how to measure various environmental factors and the growth rate and quality of vegetable and ornamental plants in a nondestructive manner using imaging and various sensors. Students will also gain practical experience in using various methods of statistical data analysis, growth model development and simulation, and environmental control. 2. Qualitative/quantitative analyses of quality and plant components (3 sessions) Students will practice extracting and analyzing plant components using such techniques as gas chromatography, high-pressure liquid chromatography, spectrophotometry, and atomic absorption photometry. Students will also conduct analyses using databases such as mass spectral databases, and examine the relationship between compounds related to the quality of vegetable and ornamental plants and genotypes or environmental conditions. 3. Analyses and evaluation of environmental stress (2 sessions) Students will learn the methods of quantifying plant reactions to environmental stress such as strong light and low temperature using chlorophyll fluorescence and growth analysis. 4. Application of tissue culture methods to seedling production (4 sessions) Students will examine efficient propagation methods using tissue culture. In addition, students will master the methods of obtaining pathogene-free plants using stem tip culture and the methods of examining the traits of cultured plant tissues. 5. Genetic analysis (4 sessions) Students will master the techniques of analyzing genomes and gene expression. In addition, they will gain practical experience in gene functional analysis by mastering gene recombination and other relevant techniques. |
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| Evaluation Methods and Policy | Grading will be based on a comprehensive assessment of attendance, commitment to projects, and mastery of techniques. | ||
| Course Requirements | Basic knowledge of vegetable horticulture, ornamental horticulture, plant physiology, plant nutrition, and soil science is desirable. | ||
| Study outside of Class (preparation and review) | Grow plant materials on a daily basis. Prepare thoroughly for laboratory experiments. In addition, summarize the methods of experiments conducted. | ||
