Laboratory Course in Aquatic Environmental Microbiology1
| Numbering Code | G-AGR04 6DC44 EJ81 | Year/Term | 2022 ・ Year-round |
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| Number of Credits | 6 | Course Type | Experiment |
| Target Year | Target Student | ||
| Language | Japanese | Day/Period | |
| Instructor name |
SAWAYAMA SHIGEKI (Graduate School of Agriculture Professor) NAKAGAWA SATOSHI (Graduate School of Agriculture Associate Professor) |
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| Outline and Purpose of the Course | There are opportunities of leaning isolation and identification of marine microbes and microalgae, feeding of microbes, measuring productivities of and carotenoids and biofuels using GC and HPLC, functional analyses of genes, and genetic recombination. | ||
| Course Goals | Understand the basic concepts related to experiments in aquatic environmental microbiology. | ||
| Schedule and Contents |
3-4 classes (total 15) will be spent on each of the following topics (instructors: Shigeki Sawayama, Satoshi Nakagawa): 1. Survey methods for the ocean, lakes and marshes Students will learn the steps involved in physicochemical research, including how to prepare a survey plan, conduct a field survey, and collect samples. Students will also learn the key concepts related to the methods of analyzing nutrient salts. 2. Methods of isolating, identifying, and quantifying microbes, algae, and symbiotic microbes Students will learn the methods of: isolating microbes and algae; identifying their genes using base sequencing; classifying them by morphological observation, and observing lipids using fluorescent staining. 3. Culture methods for microalgae, filamentous fungi, and symbiotic bacteria Students will learn the methods of culturing, isolating, and sterilizing microalgae, filamentous fungi, and symbiotic bacteria, as well as the methods of attaining optimal culture conditions and producing auxotrophs for these organisms. 4. Methods of producing valuable compounds Students will learn the methods for the production, gene analysis/manipulation, and molecular breeding of valuable compounds such as carotenoids and lipids using micro algae. Students will also learn the methods for enzymatic saccharification, ethanol fermentation, methane fermentation, and the gene analysis/manipulation and molecular breeding of filamentous fungi and yeast. Feedback: Instructors will be available to answer questions by email during the feedback period. |
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| Evaluation Methods and Policy | Refer to 'Given-year Guide to Degree Programs' for attainment levels of evaluation. | ||
| Course Requirements | Knowledge of cell biology, molecular biology, genetic engineering and microbiology is desirable. | ||
| Study outside of Class (preparation and review) | Preview and review experimental methods introduced in class. | ||
