Laboratory Course in Nutritional Science of Animals1
| Numbering Code | G-AGR04 5DC32 EJ84 | Year/Term | 2022 ・ Year-round |
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| Number of Credits | 6 | Course Type | Experiment |
| Target Year | Target Student | ||
| Language | Japanese | Day/Period | |
| Instructor name |
FUNABA MASAYUKI (Graduate School of Agriculture Professor) TOMONAGA SYOUZOU (Graduate School of Agriculture Assistant Professor) |
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| Outline and Purpose of the Course | This course introduces and shows how to quantify vitamins, minerals, hormones and growth factors in blood and tissues. Through this course, participants are expected to learn analyses and interpretation of blood and tissue concentrations of these elements and bioactive substances. | ||
| Course Goals | Students will be able to not only gain proficiency in specialized analytical techniques, but also learn new techniques more easily by understanding the principles underlying analytical methods in general. | ||
| Schedule and Contents |
Students will conduct experiments in sessions 1 to 29 on the following topics: The 30th session will be set aside for questions and feedback. 1) Measurement of mineral concentrations in blood and organs (sessions 1 to 5: Matsui) Students will learn how to prepare samples and measure mineral concentrations using atomic absorption spectrometry, colorimetric methods, and the ICP-MS method. 2) Measurement of vitamin concentrations in blood (sessions 6 to 10: Matsui) Students will learn how to prepare samples and measure vitamin concentrations using high-performance liquid chromatography (HPLC). 3) Exhaustive measurement of metabolite concentrations in blood and organs (sessions 11 to 20: Tomonaga) Students will learn how to prepare samples and take an exhaustive measurement of metabolites using the GC-MS technique. 4) Measurement of the expression of hormones and growth factors in organs (sessions 21 to 29: Funaba) Students will learn how to prepare samples and measure the expression of hormones and growth factors using the Western blot technique and the RT-PCR technique. |
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| Evaluation Methods and Policy |
Class performance and a report submitted at the end of the second semester. Refer to 'Guide to Degree Programs' for attainment levels of evaluation. |
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| Course Requirements | None | ||
| Study outside of Class (preparation and review) | Go through the protocol for conducting analysis and visualize each step in advance. Review the important points of the analysis. | ||
