Laboratory Course in Animal Physiology and Anatomy2

Numbering Code G-AGR04 6DC35 EJ84 Year/Term 2022 ・ Year-round
Number of Credits 6 Course Type Experiment
Target Year Target Student
Language Japanese Day/Period
Instructor name ohta takeshi (Graduate School of Agriculture Professor)
SUGIMOTO MIKI (Graduate School of Agriculture Assistant Professor)
Outline and Purpose of the Course This course aims to assess the physiological functions of animals at the molecular, cellular, tissue and whole-body levels.
Course Goals Understand the basic concepts related to the anatomy of mammals and the methods of conducting physiological and biochemical research on their tissues and cells.
Schedule and Contents Students will conduct experiments on the following topics:

1. Anatomical experiments on mammals (sessions 1 to 4: Shinichi Kume, Miki Sugimoto, Shuntaro Ikeda)
  Students will gain experience in dissecting various domestic and laboratory animals and learn how to remove organs, observe and record data with the naked eye, and conduct a blood component analysis.

2. Biochemical experiments on tissues and cells (sessions 5 to 8: Shinichi Kume, Miki Sugimoto, Shuntaro Ikeda)
  Students will learn the methods of fixing, dehydrating, embedding, freezing and slicing organs, and conducting biochemical experiments on tissues and cells using optical and electron microscopes.

3. Histomorphological experiments (sessions 9 to 12: Shinichi Kume, Miki Sugimoto, Shuntaro Ikeda)
  Students will gain experience in enzyme-histochemical methods, fluorescent antibody techniques, the enzymatic method for immunohistochemistry, and in situ PCR methods for locating proteins and nucleic acid in tissues and cells.

4. Experiments on the environmental physiology of ruminants (sessions 13 to 15: Shinichi Kume, Miki Sugimoto, Shuntaro Ikeda)
  5. Investigate the physiological mechanisms in cows such as body temperature and pulse, and the relationship between their blood biochemical, endocrine, and immune components and environmental factors such as ambient temperature and humidity, and analyze the impact of environmental factors on the physiological functions of animals.
Evaluation Methods and Policy Evaluation will be based on reports and attendance.Refer to '2016 Guide to Degree Programs' for attainment levels of evaluation
Course Requirements Basic knowledge of animal physiology, molecular biology, environmental physiology, and anatomy.
Study outside of Class (preparation and review) Students are expected to review their knowledge of mammalian anatomy and the physiological and biochemical research methods for mammalian tissues and cells.
PAGE TOP