Laboratory Course for Reproductive Biology2

Numbering Code G-AGR04 5DC31 SB84 Year/Term 2022 ・ Year-round
Number of Credits 6 Course Type Experiment
Target Year Target Student
Language Japanese Day/Period
Instructor name MINAMI NAOJIROU (Graduate School of Agriculture Professor)
IKEDA SHUNTAROU (Graduate School of Agriculture Associate Professor)
HONDA SHINNOSUKE (Graduate School of Agriculture Assistant Professor)
Outline and Purpose of the Course Special tequniques and methodologies in the field of Reproductive Biology are introduced. This course is an advansed one of Laboratory Course for Reproductive Biology 1.
Course Goals To establish special technology, methodologies, and experimental planning in the field of Reproductive Biology.
Schedule and Contents Special scientific topics are related to in vitro fertilization of mammalian eggs, embryo culture, embryonic manipulation for nuclear transfer and microinjection, cell culture of pluripotent stem cells and germ cells

Technical issues related to cellular reprogramming, embryonic development and gene regulation of early embryonic development are mainly introduced in this program.
1. Germ cell differentiation and maturation (15 weeks: Imai)
2. Molecular biology in fertilization and embryonic development in mammals (15 weeks: Minami).
3. Oocyte maturation and embryonic development in vitro (15 weeks: Yamada)
4. Embryonic stem cells, male germ cells, and regulation of cell differentiation (15 weeks: Imai)
5. Production of genetic modified animals and application to animal breeding (15 weeks: Minami)
6. Efficient production of nuclear transferred animals and mechanisms of nuclear reprogramming (15 weeks: Yamada)
7. A feedback will be informated in this course.
Evaluation Methods and Policy The point of reference will be the number of attendance in the course.
Refer to '2017 Guide to Degree Programs' for attainment levels of evaluation.
Course Requirements None
Study outside of Class (preparation and review) Preliminary knowledge regarding reproductive biology, developmental biology, and molecular biology are required.
Textbooks Textbooks/References Not used.
References, etc. Announced in the course.
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