Laboratory Course in Tree Cell Biology1
| Numbering Code | G-AGR02 5BC38 EJ80 | Year/Term | 2022 ・ Year-round |
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| Number of Credits | 5 | Course Type | Experiment |
| Target Year | Target Student | ||
| Language | Japanese | Day/Period | |
| Instructor name |
SUGIYAMA JUNJI (Graduate School of Agriculture Professor) YOSHINAGA ARATA (Graduate School of Agriculture Associate Professor) AWANO TATSUYA (Graduate School of Agriculture Assistant Professor) |
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| Outline and Purpose of the Course | Newly developed fixation methods, such as high pressure freezing, are applied to differentiating xylem of angiosperm and gymnosperum to fix the cells in living state. Immunolabeling of hemicelluloses and lignin are applied to the differentiating xylem to observe their deposition and distribution. | ||
| Course Goals |
?Develop the ability to conduct research independently by mastering a wide range of experimental methods for investigating lignified cell walls; ?Develop the ability to conduct research independently by mastering a wide range of experimental methods for investigating the cell wall during its formation. |
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| Schedule and Contents |
3-5 weeks of class are planned for each of the topics covered in this course, including the following: 1. Electron microscopy Students will learn the basic principles and applications of ultrastructural investigation methods using transmission and scanning electron microscopes. 2. Image processing Students will learn the techniques for conducting two-dimensional and three-dimensional analyses of cell morphology by using a standard computerized image processing method and Fourier Transform. 3. Diffraction methods Students will learn the common principles of X-ray diffraction, electron diffraction, and optical diffraction, their individual characteristics and practical applications. 4. Observation methods for cambium activity Students will learn the methods of collecting and fabricating samples for tracking the processes of cell division and production and xylem differentiation in trees, primarily by using optical microscopes. 5. Immunohistochemical tracking methods Students will learn the basics and applications of immunohistochemical staining, a method used to visualize the locations of cell wall components and the enzymes related to their synthesis. 6. Localization of cell wall components Students will learn the principles of methods such as microspectrophotometry, fluorescent photometry, and component analysis methods for extremely small samples, and problems associated with the application of such methods. 7. Freezing and observation methods for storing ultrastructures in their living state Students will learn the principles and techniques of rapid-freeze methods and freeze-replica methods to store a variety of cell organelles in good condition. |
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| Evaluation Methods and Policy |
Evaluation will be based on attendance. Refer to '2017 Guide to Degree Programs' for attainment levels of evaluation. |
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| Course Requirements | Background knowledge: optics, X-ray diffraction, biochemistry, botany, and cytology. | ||
| Study outside of Class (preparation and review) | Students will conduct experiments while consulting with their supervisors. | ||
