Numbering Code	G-AGR03 6CA22 LJ29	Year/Term	2022 ・ Intensive, First semester
Number of Credits	1	Course Type	Lecture
Target Year	2nd year master's students	Target Student
Language	Japanese	Day/Period	Intensive
Instructor name	YAZAKI KAZUFUMI (Research Institute for Sustainable Humanosphere Professor) SUGIYAMA AKIFUMI (Research Institute for Sustainable Humanosphere Associate Professor)
Outline and Purpose of the Course	Basic points of plant molecular biology and cell biology are reviewed. Taking plant-specific metabolism, e.g. secondary metabolism as an example, biosynthetic enzymes and transporters for functional small molecules are lectured. Also, biotechnological applications of useful genes to alter plant functions are also introduced.
Course Goals	Acquire the basic concepts related to functional low-molecular organic compounds, including their roles in plants, biosynthesis, and the mechanisms of accumulation, and understand how they relate to our lives.
Schedule and Contents	Gene expression control in plant cells and tissues 　　The sessions will outline the cell biology and molecular biology of vascular plants to provide students with an understanding of the characteristics of vascular plants in comparison to those of animals. A comprehensive overview will be presented using examples such as metabolic enzyme genes for functional low molecules, as exemplified by secondary metabolites, and transporter genes such as ABC transporters. In addition, control mechanisms for cell-specific and tissue-specific gene expression will be explained. Manifestation of foreign genes in plants 　　Topics related to plant biotechnology, such as production of valuable compounds by modifying flower color and nutritional value, improvement of salt tolerance, and plants related to environmental cleanup and conservation. Basic technologies, necessary specialized knowledge and key techniques will be discussed. Basics of symbiotic nitrogen fixation by plants, primarily by leguminous plants, and membrane transporters 　　We will discuss symbiotic nitrogen fixation, which plays an important role in nitrogen cycle on the planet. Other topics include a highly regulated nodulation process that occurs between root nodule bacteria and plants and the physiological roles of membrane transporters related to the functions of the process, with a focus on leguminous plants The course will be held over two days on a Monday and a Tuesday from 3rd to 6th period. On the last day, the 5th period will be set aside for questions and the instructor will be available in his office to answer questions from students.
Evaluation Methods and Policy	Evaluation will be based on reports and attendance.
Course Requirements	Previous experience in basic experiments in organic chemistry, biochemistry, molecular biology, and cellular biology is desirable, but not required.
Study outside of Class (preparation and review)	Students need to preview and review key concepts in biochemistry, plant cellular biology, and organic chemistry, which are required for this course.