Life Sciences Ⅲ (Advanced Course)
| Numbering Code | G-AGR03 6CA26 LJ79 | Year/Term | 2022 ・ Intensive, Second semester |
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| Number of Credits | 1 | Course Type | Lecture |
| Target Year | Target Student | ||
| Language | Japanese | Day/Period | Intensive |
| Instructor name |
TEI HAJIME (Part-time Lecturer) INOUE YOSHIHARU (Graduate School of Agriculture Professor) |
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| Outline and Purpose of the Course | Koji-molds including Aspergillus oryzae have been employed for manufacturing Japanese traditional fermented products such as sake, shoyu (soy sauce), and miso (soybean paste) etc. over a thousand years, and thus they are the most industrially important microbes in Japan. Elucidation of various life phenomenon that they show is very important issues from the points of view of both academia and industry. In this lecture, recent advances in the study on regulatory mechanisms for industrially important gene expression in Aspergillus oryzae will be introduced. This lecture helps students to understand fungal gene expression regulatory mechanisms in filamentous fungi including Aspergillus oryzae as well as to deepen understanding about the industrial significance of the gene regulation research. | ||
| Course Goals |
Students will aim to: Understand recent advances in research concerning regulatory mechanisms for fungal gene expression. Understand the characteristics of methods for gene expression analyses. Understand the variety of the gene expression manners in various culture environments. Understand the significance of transporters that affect the gene expression and the regulatory protein degradation mechanism for transporters. Understand the high-level production of industrially important enzymes through the artificial modification of regulatory machineries of gene expression, and thereby better understand the industrial significance of the gene regulation research. |
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| Schedule and Contents |
1. Regulatory mechanisms for amylolytic gene expression in Aspergillus oryzae 2. Regulatory mechanisms for genes specifically expressed in solid-state culture in Aspergillus oryzae 3. Properties of carbon catabolite repression machineries and high-level production of hydrolytic enzymes by means of down-regulation of such machineries 4. Regulatory protein degradation of maltose transporter in Aspergillus oryzae 5. Molecular characterization of the fungal transcription factor involved in azole drug resistance |
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| Evaluation Methods and Policy | Students are evaluated on their points from their class attendance and submitted reports. | ||
| Course Requirements | None. | ||
| Study outside of Class (preparation and review) | Student will understand easily the content of the lecture if they read in advance the corresponding chapters in textbooks or booklets for genetic engineering and molecular biology. | ||
