Applied Structural Biology
| Numbering Code | G-AGR03 6CA15 LJ79 | Year/Term | 2022 ・ Intensive, First semester |
|---|---|---|---|
| Number of Credits | 1 | Course Type | Lecture |
| Target Year | 2nd year master's students | Target Student | |
| Language | Japanese | Day/Period | Intensive |
| Instructor name | MIZUTANI KIMIHIKO (Graduate School of Agriculture Assistant Professor) | ||
| Outline and Purpose of the Course | In this lecture, we will give an introduction of the practical ways of protein x-ray crystallography, including crystallization, diffraction data collection, structure determination and structure refinement. Students will have experiences about crystallization, data collection and computer graphics. Using lysozyme as a model protein. | ||
| Schedule and Contents |
Students will aim to: Learn about protein crystallization and X-ray crystallography, and gain a good understanding of research papers on structural biology in which X-ray crystallography is used; Understand the practical applications of X-ray crystallography and be able to conduct experiments and perform calculations independently. The course will cover the following topics: a. Protein crystallization b. Crystallographic properties c. X-ray diffraction d. Structure determination methods (heavy atom replacement method, molecular replacement method, etc.) e. Molecular modeling and structural refinement f. Use of synchrotron radiation g. Functional conversion using protein engineering The course will be held on two Mondays from 3rd to 6th period. |
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| Evaluation Methods and Policy | Evaluation will be based on attendance and reports. | ||
| Course Requirements | General knowledge of proteins is required. | ||
