Forest Molecular Biology
| Numbering Code | U-AGR05 4E133 LJ80 | Year/Term | 2022 ・ First semester | |
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| Number of Credits | 2 | Course Type | Lecture | |
| Target Year | 4th year students | Target Student | ||
| Language | Japanese | Day/Period | Tue.2 | |
| Instructor name |
HONDA YOICHI (Graduate School of Agriculture Professor) KAWAUCHI MORIYUKI (Graduate School of Agriculture Program-Specific Associate Professor) |
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| Outline and Purpose of the Course | Recently, molecular biological research methods have become popular as approaches used in the elucidation of mechanisms from production to decomposition of biomass by forest organisms, molecular breeding, diversity analysis, etc. In this lecture, we will learn the principles of the main genome and genetic engineering methods used in the field of forest science, including examples of their utilization and their future. | |||
| Course Goals | Students who want to use genetic engineering, genome engineering, etc. for future forest biology research will study the types and principles of general methodologies, and solidify the foundations, with the aim of enabling them to singlehandedly formulate experiment methods in response to various research issues. | |||
| Schedule and Contents |
Small interactive classes are planned for about one to three weeks per topic for the following topics. First session: Biomass and genetic engineering What is forest molecular biology? Genes and their products responsible for the production and decomposition of biomass. What can we know and do with genetic engineering? Second to fourth Sessions: DNA analytical methods (PCR, DNA markers, environmental DNA, etc.) Species identification and analysis of difficult-to-cultivate organisms, such as endophytes and lichens. Fifth to seventh sessions: Gene cloning and transformation methods (vectors, recombinant DNA, transformation, hybridization, etc.) Genetically engineered wood: Can natural polymers be designed? Eighth and ninth sessions: Gene function analytical methods (expression analysis, gene knockout [genetic destruction], gene knockdown [antisense RNAs, RNAi], antibody analysis, artificial mutual introduction, etc.) What is the true culprit of lignin degradation? Tenth and eleventh sessions: Genome analysis (genome analysis, database and in silico analysis, genomic engineering, etc.) Examples of genome analysis of mushrooms and trees. Twelfth and thirteenth sessions: Post-genome analysis (transcriptome, proteome, metabolome analysis, etc.) Understand wood decomposition as a system. Fourteenth session: Future of forest molecular biology Understanding the logic of nature, learning naturally, and coexisting with nature. Fifteenth session: Feedback The instructor will be available in the laboratory to answer questions from students. |
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| Evaluation Methods and Policy |
Evaluation standards and policies are based on those described in the Faculty of Agriculture Handbook for the relevant fiscal year. Evaluation is based on proactive participation in classes (70%) and degree of understanding of class content (30%). |
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| Course Requirements | Forest Biochemistry I and II must be completed in advance. It is assumed that students will have basic knowledge of general biochemistry and molecular biology, such as the structure of nuclear acids, and the mechanism of transfer and translation. | |||
| Study outside of Class (preparation and review) | Review textbooks weekly, according to the shared schedule. | |||
| Textbooks | Textbooks/References | Takaaki Tamura 基礎から学ぶ遺伝子工学 (Yodosha) (ISBN: 978-4-7581-2083-8) Since this explains the relevant sections in a seminar format, the participants should be prepared. The list price is 3400 yen plus tax, and it is also available at the Co-Op. | ||
| References, etc. | Yosuke Ejima, "これだけは知っておきたい図解分子生物学" (Ohmsha) ISBN: 978-4274201172) | |||
