京都大学 京都大学

Applied Microbiology III

Numbering Code U-AGR02 3B128 LJ79 Year/Term 2022 ・ Second semester
Number of Credits 2 Course Type Lecture
Target Year 3rd year students Target Student
Language Japanese Day/Period Wed.2
Instructor name OGAWA JIYUN (Graduate School of Agriculture Professor)
YURIMOTO HIROYA (Graduate School of Agriculture Associate Professor)
Outline and Purpose of the Course Students will acquire basic knowledge about the major metabolic pathways (e.g., sugars, organic acids, amino acids, nucleic acids, and lipids) that form the basis of microbial function and the biosynthetic pathways of secondary metabolites (e.g., antibiotics) and their industrial uses. They will also learn about genetic engineering, enzyme engineering, molecular breeding, and cell engineering which is necessary for designing and controlling microbial functions.
Course Goals Students will learn the basics of substance production technology through fermentation production, microbial conversion, enzyme conversion, etc. They will gain a deep understanding of substance metabolism, enzyme reaction, gene manipulation technology, and microbial breeding method and acquire the background to design the substance production process that uses the microbial function.
Schedule and Contents We plan to give 1-2 weeks of lessons per topic for the following topics.

1) Metabolic pathway and substance production of carbon compounds in microorganisms: I (Ogawa)
2) Metabolic pathway and substance production of carbon compounds in microorganisms: II (Ogawa)
3) Metabolic pathway and substance production of carbon compounds in microorganisms: III (Ogawa)
4) Fermentation and conversion of fats and oils (Ogawa)
5) Microbial metabolism in the carbon cycle in the environment (Yurimoto)
6) Microbial metabolism in the nitrogen cycle in the environment (Ogawa)
7) Metabolic pathway and substance production of nitrogen compounds in microorganisms: I (Ogawa)
8) Metabolic pathway and substance production of nitrogen compounds in microorganisms: II (Ogawa)
9) Establishment of controlled fermentation (Introduction to amino acid and nucleic acid fermentation and mutation and breeding method) (Yurimoto)
10) Amino acid fermentation details: I (Ogawa)
11) Amino acid fermentation details: II (Ogawa)
12) Nucleic acid fermentation details: I (Ogawa)
13) Nucleic acid fermentation details: II (Ogawa)
14) Fermentation production and conversion of secondary metabolites (Ogawa)
[Final exam]
15) Feedback: At the feedback time, students will meet in the classroom or laboratory to have their questions answered.
Evaluation Methods and Policy Exam results (90%) and assignment reports (10%)
The evaluation criteria and policies are based on those described in the Student Handbook of the Faculty of Agriculture for the relevant academic year.
Course Requirements Recommended: Introduction to Applied Life Science II and Applied Microbiology I/II
Study outside of Class (preparation and review) Prepare and review the distributed teaching materials.
Textbooks Textbooks/References 横田　篤、大西康夫、小川　順 『応用微生物学第３版』 (文永堂出版), prints will be distributed.
References, etc. 熊谷英彦ら 『遺伝子から見た応用微生物学』 (朝倉書店), 大嶋泰治ら 『IFO微生物学概論』 (培風館)
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