Experimental Course of Plant Gene Expression2
| Numbering Code | G-AGR03 6CC44 EJ29 | Year/Term | 2022 ・ Year-round |
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| Number of Credits | 6 | Course Type | Experiment |
| Target Year | Target Student | ||
| Language | Japanese | Day/Period | |
| Instructor name |
YAZAKI KAZUFUMI (Research Institute for Sustainable Humanosphere Professor) SUGIYAMA AKIFUMI (Research Institute for Sustainable Humanosphere Associate Professor) MUNAKATA RYOSUKE (Research Institute for Sustainable Humanosphere Assistant Professor) |
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| Outline and Purpose of the Course | Basic techniques for plant molecular biology and biochemistry are taught. Taking one or a couple of genes, especially those coding for useful metabolic enzymes and transporter proteins, as target gene, molecular cloning, functional expression, enzyme activity mesurements, transport assay, and physiological roles of those genes are studied. Biotechnological applications are also tried using those genes. Recently, regarding nitrogen fixiation studies, legume plant and nodule-specific genes are also dealt with. | ||
| Schedule and Contents |
Experiments involve following research topics: Analysis of gene expression and function of gene products in various economic plants. Molecular breeding of model plants using genes encoding biosynthetic enzymes and transporter proteins from various plant species. Membrane transport of functional small molecules, such as plant hormons, alkaloids, phenolic compounds, and organic acids. Analysis of genes involved in symbiotic nitrogen fixiation and the formation of nodules. Interaction of root exudates and microbial flora in rhizosphere is also studied. Applied researches using useful genes for sustainable agriculture. |
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| Evaluation Methods and Policy | Based on the ability to proceed with biochemical and molecular biological experiments, quality of data, ability to summarize data, interpretation of data, flaxibility to overcome troubles, quality of discussion, and wrting skill of publication. | ||
| Course Requirements | Basic knowledge of biochemistry, molecular biology, and natural product chemistry. | ||
| References, etc. | Based on the ability to proceed with biochemical and molecular biological experiments, quality of data, ability to summarize data, interpretation of data, flaxibility to overcome troubles, quality of discussion, and wrting skill of publication. | ||
