Applied Microbiology II
| Numbering Code | U-AGR02 3B127 LJ79 | Year/Term | 2022 ・ First semester | |
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| Number of Credits | 2 | Course Type | Lecture | |
| Target Year | 3rd year students | Target Student | ||
| Language | Japanese | Day/Period | Tue.2 | |
| Instructor name |
INOUE YOSHIHARU (Graduate School of Agriculture Professor) YURIMOTO HIROYA (Graduate School of Agriculture Associate Professor) |
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| Outline and Purpose of the Course | Lectures will be given on the basics of breeding and its application examples from the standpoints of microbial genetics, biochemistry, molecular biology, and molecular engineering to deepen understanding of biotechnology that uses microbial metabolic pathways and enzymes to link practical microbial breeding and substance production. | |||
| Course Goals | Students will learn the basics of microbiology, which are necessary for understanding microbial breeding methods and genetic recombination technology, and will be able to apply it to biotechnology. | |||
| Schedule and Contents |
Lectures will be given on each of the following items. For each item, the number of weeks specified in [brackets] will be allocated while confirming the degree of understanding among the students. The order of lectures for each item and sub-item and the number of lecture weeks allocated to each are not fixed; the lecturer will decide what is appropriate according to the lecture policy of the person in charge and the students' academic background and understanding. Appropriate instructions will be given on how to proceed with the lecture, and due consideration will be given so the students can prepare and review. (1) Basics of genetics [1 week]: Mendelian inheritance, basics of microbial genetics (Inoue) (2) Microbial genome [2 weeks]: DNA and genome structure, DNA replication, genome inheritance, etc. (Inoue) (3) Genetics of prokaryotic microorganisms [3 weeks]: Mutations and their repair, complementation and suppression, recombination, extranuclear genes, genetics of phages and viruses, etc. (Inoue) (4) Expression of microbial genes [2 weeks]: Transcription and translation control mechanism of prokaryotic and eukaryotic microorganisms (Inoue) (5) Basics of gene recombination technology [2 weeks]: Restriction modification system, restriction enzyme, ligase, host-vector system, PCR, genome editing technology, etc. (Inoue) (6) Genetics of eukaryotic microorganisms [2 weeks]: Genetics of yeast and mold (Yurimoto, Inoue) (7) Application of gene recombination technology [2 weeks]: Production of heterologous proteins by yeast, control of the metabolic system and substance production by gene recombination, etc. (Inoue, Yurimoto) [Final exam] (8) Feedback [1 week] |
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| Evaluation Methods and Policy |
Evaluation method: Regular test: 60%, General scores: 40% General score evaluation (degree of participation in class) includes evaluation of attendance status and quizzes imposed irregularly. Evaluation criteria: The evaluation criteria and policies are based on the evaluation criteria and policies described in the Student Handbook of the Faculty of Agriculture for the relevant academic year. |
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| Course Requirements | Recommended: Applied Microbiology I | |||
| Study outside of Class (preparation and review) | Prepare and review the distributed materials and reference books introduced during class. | |||
| Textbooks | Textbooks/References | In addition, prints, etc., will be distributed as appropriate. | ||
| References, etc. | 中村千春 『遺伝学』 (化学同人), 阪井康能ら 『遺伝子・細胞から見た応用微生物学』 (朝倉書店) | |||
